osteogenic differentiation media  (STEMCELL Technologies Inc)

Journal: NPJ Regenerative Medicine

Article Title: Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing

doi: 10.1038/s41536-025-00408-z

Figure Lengend Snippet: A Representative overlayed images depicting RUNX2 expression in MSCs that underwent osteogenic differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.

Article Snippet: MSCs were seeded at 2 × 10 4 /cm 2 for each well and allowed to grow to confluency before changing to osteogenic differentiation media (Lonza).

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice

doi: 10.1186/s13287-025-04145-4

Figure Lengend Snippet: HUCMSCs ameliorated OVA-induced airway inflammation in mice through migrasomes. A HUCMSCs of wild type and TSPAN4-KD were stained with 1 µg/ml WGA (Alexa 488) and observed under confocal microscopy. Scale bar = 10 μm. B Western blot analysis of TSPAN4 expression in hUCMSCs of wild type and TSPAN4-KD. C Experimental protocol for the development of murine allergic asthma model. D Representative images of lung sections stained with HE (up) and PAS (down) from each group are presented (black bar = 100 μm). E The inflammatory infiltration and goblet cell hyperplasia were quantified by HE (left) and PAS (right) scores ( n = 6). F Statistical analysis was conducted on the total inflammatory cells (left) and eosinophils (right) in the BALF ( n = 6). Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was performed on the data. * P < 0.05, ** P < 0.01, *** P < 0.001. Full-length blots are presented in supplemental Figure

Article Snippet: HUCMSCs and hUCMSC TSPAN4−KD were cultured in OriCellTM hUCMSCs osteogenic differentiation media (Cyagen) as described by manufacturer.

Techniques: Staining, Confocal Microscopy, Western Blot, Expressing